In the base case, the risk that a child would develop mild, severe, and fatal EV71-HFMD between age 6 mo and 5 y was 3,088 (95% CI 3,084–3,093), 83.4 (95% CI 82.6–84.2), and 3.1 (95% CI 3.0–3.3) per 100,000, respectively (Fig 3A; Table 1). Xing, W. We found that HFMD occurrences were generally more sensitive to temperature effects at lower latitude, but more sensitive to relative humidity effects at higher latitude. During 2008 to 2012, EV71 and CVA16 were the most predominant causative agents for HFMD, while in 2013, the major causative agent was CVA6. For non-EVA71 and non-CVA16 enterovirus, CVA6-specific real-time RT-PCR was performed by using commercial kit (JC20106, bioPerfectus technologies, Jiangsu, China). What we can do is to report what we have actually found, better in detailed content and some known preventive knowledge to the public. By comparing the value of PD of D1, D2 and E, we were able to determine the influence of the intersection [15,16].

At the region of 2A, Chinese C4 viruses were phylogenetically closest to C genotype strains and CVA-4 prototype strain; at 2B region, C4a, C4b viruses closest to CVA-4; This indicated that 2A, 2B region was a genomic region of multi-mutation and a breakpoint for the recombination; while at 2C,3A,3B region, similar phylogeny pattern was shared between C4a, C4b viruses and other HEV-A strains: C4a, C4b viruses were closest to B0-5 subgenotype strains, and trended to closer to CVA-16, CVA-14, CVA-4; At 3C, 3D and 3′UTR region, the C4a, C4b viruses apparently to be closer to CVA-16, CVA-14,CVA-4 than the subgenotypes strains of B genotype, except for B3 subgenotype. Full genomes for each of the four serotypes (A2SD09, A4SZ09, A5SD09, and A10SD09) were completed. Two phylogenetic trees were constructed by the VP1 nucleotide sequences of CV-A10 and CV-A6 from the present study and those downloaded from GenBank, respectively (Figure 4 and Figure 5). EV71 A3 or A8-specific CD4+ T cells respond to the poliovirus 3 Sabin (PV3) homolog, A3v peptide. Time series plot of weekly HFMD cases and climatic variables of China, 2008–2013. J Virol 87, 7637–7645, doi: 10.1128/jvi.03519-12 (2013). 48, Issue.


The weeks of lag effects were selected according to the related literatures and statistically significant lag effects terms tested by Polynomial distributed lag model. Moreover, the phylogenetic analysis also suggested that intratypic recombination may occur among EV-C96 strains. Infectivity titer, described as tissue culture infectious dose 50 (TCID50), of the virus in MA104 cells (1×105 cells/well) was performed following the previously described method of Reed and Muench (26). Studies are underway in Vietnam to assess the efficacy of magnesium sulfate as a therapeutic intervention, to identify genetic and antigenic evolution of the virus, to look at host genetic factors associated with severe disease, and to evaluate clinical predictors of disease progression and neurodevelopmental complications of severe disease. PCR primers were designed separately for each gene using Primer Premier 5 software or as previously described (Table 1) [21]. Shanxi HFMD data were provided by the Shanxi Center for Disease Control and Prevention and were obtained from the National Surveillance System. After PCR, genotypes were determined by sequencing.

The extracted RNAs were reverse-transcribed using random hexamers. Such seasonal distribution suggests climatic variations may play a certain role in the transmission of the disease, particularly in temperate areas. Most patients recover within 7–10 days without any clinical attention due to its typically mild and self-limiting nature. A. More than one hundred serotypes have been identified in the genus Enterovirus [2], and classified into twelve species. The dominant strain is Coxsackievirus A16 (CA 16) and Enterovirus A71 (EV 71). This was contrary to a previous study, which suggested more common EV71 infections in South China in 2005 [42].

Unfortunately, no treatment for HFMD is available yet. Copyright: © Mao et al. Children in Beijing are suffering from an outbreak of mouth sores caused by enterovirus, similar to hand foot and mouth disease. These data indicate that a major cytokine response and inflammation, in both plasma and the CNS, are features of disease caused by EV71 infection. The effects peaked at 30.5 °C (the 98th percentile of temperature), which was similar to the finding in Japan (about 29 °C, the 95th percentile of temperature) [22]. Inter-typic recombinant EVs were reported to cause severe or fatal cases of HFMD10,11; however, there was no clear evidence that inter-typic recombinant EVs had previously contribute to outbreaks of HFMDs5,12. EV71 infection was mainly popular in 2008.

I think even in the modern society, this is still the highest realm of medical treatment. 2014 L00188). Fengcai Zhu from Jiangsu provincial center for disease prevention and control and his colleagues have completed the phase III evaluation of the EV71 vaccine for the first time globally, and found that EV71 vaccine provides high efficacy, satisfactory safety, and sustained immunogenicity. Hand, foot, and mouth disease (HFMD) has been emerging as an important public problem over the past few decades, especially in Asian and Pacific regions.